IN VITRO ANTIBACTERIAL ACTIVITY OF WAX APPLE LEAF EXTRACT (Syzygium samarangense) AGAINST SEVERAL PATHOGENIC STRAINS OF VIBRIO

Abstract

The purpose of this study is to determine the antibacterial activity of wax apple leaf extract (WALE) against several pathogenic Vibrio strains. Antibacterial activity, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) of wax apple leaf extract were examined in Vibrio parahaemolyticus, Vibrio vulnificus, Vibrio alginolyticus, and Vibrio harveyi strains. The results
showed that the zones of bacterial inhibition against Vibrio parahaemolyticus, Vibrio vulnificus, Vibrio alginolyticus, and Vibrio harveyi were 16.00±0.00 mm, 16.67±0.58 mm, 20.00±0.00 mm, 17.00±0.00 mm, respectively. The highest minimum inhibitory concentration and minimum bactericidal concentration values of wax apple leaf extract (2,500 and 5,000 µg/mL) were determined for V. harveyi while the lowest minimum inhibitory concentration (156.25 µg/mL) and minimum bactericidal concentration values (312.5 µg/mL) were found for V. vulnificus. The minimum inhibitory concentration and minimum bactericidal concentration values were observed to be 1,250 µg/mL and 2,500 µg/mL for V. parahaemolyticus; doubled that of minimum inhibitory concentration (625 µg/mL) and minimum bactericidal concentration (1,250 µg/mL) for V. alginolyticus. The wax apple leaf extract was proven to have antibacterial activity against four strains of Vibrio above, with a ratio of MBC/MIC = 2.0.