EFFECTS OF Bacillus amyloliquefaciens D19 CULTURE CONDITIONS ON IN-VITRO ANTIFUNGAL ACTIVITY AGAINST Neoscytalidium dimidiatum

Authors

  • Ngoc An Nguyen Industrial University of Ho Chi Minh City, Vietnam
  • Phuc Thanh Nguyen Industrial University of Ho Chi Minh City, Vietnam
  • Truong Chinh Hua Industrial University of Ho Chi Minh City, Vietnam
  • Hanh Thi Dieu Nguyen Industrial University of Ho Chi Minh City, Vietnam
  • Tan Viet Pham Industrial University of Ho Chi Minh City, Vietnam

DOI:

https://doi.org/10.35382/tvujs.14.3.2024.7

Keywords:

antifungal, Bacillus amyloliquefaciens, culture condition, dragon fruit, Neoscytalidium dimidiatum

Abstract

The fungus Neoscytalidium dimidiatum is a pathogen that causes diseases in a variety of plants, with a particular preference for dragon fruit. In the search for safe and effective control of N. dimidiatum diseases in dragon fruit, the focus has been directed towards research of biological controls. This study investigates the Bacillus amyloliquefaciens D19 culture conditions for favorable antifungal
activity against N dimidiatum. The highest antifungal activity was achieved in culture maintained at 45oC with agitation at 150 rpm
for 21 hours in the defined medium comprised of 0.5 g/L MgSO4.7H2O, 3.0 g/L NaH2PO4.2H2O, 3.0 g/L Na2HPO4.12H2O, 10 g/L sucrose, and 10 g/L peptone with initial pH 6.0. Additionally, the antifungal activity of the bacterial culture was found to be stable at temperatures less than 60oC and pH conditions ranging from 7.0 to 9.0. Overall, the findings of this study lay a foundation for future research into the application of biological antagonists in the control of N. dimidiatum diseases in plants.

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Published

30-09-2024

How to Cite

Ngoc An Nguyen, Phuc Thanh Nguyen, Truong Chinh Hua, Hanh Thi Dieu Nguyen, & Tan Viet Pham. (2024). EFFECTS OF Bacillus amyloliquefaciens D19 CULTURE CONDITIONS ON IN-VITRO ANTIFUNGAL ACTIVITY AGAINST Neoscytalidium dimidiatum. TRA VINH UNIVERSITY JOURNAL OF SCIENCE, 14(3). https://doi.org/10.35382/tvujs.14.3.2024.7

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