CHARACTERISTICS OF THE STRAIN Lactobacillus plantarum UL487 ISOLATED FROM TOFU (HUE)
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Published:
Dec 30, 2020
Keywords:
antibacterial activity, bacteriocin, cold shock protein, lactic acid bacteria, Lactobacillus plantarum
Main Article Content
Abstract
Lactobacillus plantarum is widely used in many fields, especially in medicine. In this study, besides the
strain’s morphology (i.e, the colony and the cell under microscrope and scan electronic microscope), antibacterial activities against some pathogenic bacteria and the nucleotide sequences encoded for bacteriocin PlnA, PlnEF and the cold shock protein (Csp) of L. plantarum UL487 isolated form Tofu (Hue) were studied
by agar diffusion method and cloning. The results showed that: i) the morphology of the colony and the cell of the strain were typical for lactic acid bacteria; ii) UL487 owned antibacterial activities against some pathogenic bacteria such as Staphylococcus aureus VTCCB 658, Bacillus cereus VTCCB 613 and Salmonella enterica VTCCB 480; and iii) the nucleotide sequences encoded for bacteriocin PlnA, PlnEF and the amino acid of the cold shock protein (Csp) of the strain were 96%, 99% and 94% identical to those of the L. plantarum in the Gen Bank, respectively.
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1.
Nguyen D, Hoang V, Nguyen U. CHARACTERISTICS OF THE STRAIN Lactobacillus plantarum UL487 ISOLATED FROM TOFU (HUE). journal [Internet]. 30Dec.2020 [cited 2May2024];10(40):142-8. Available from: https://journal.tvu.edu.vn/index.php/journal/article/view/625
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References
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cold shock protein of the strain Lactobacillus plantarum UL485 isolated from chao of
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2011; 1(3):1–13.
[2] De Vuyst L, Leroy F. Bacteriocins from lactic acid bacteria: Production, purification,
and food applications. Journal of Molecular Microbiology and Biotechnology. 2007;
13(4):194–199.
[3] Diep D. B, Myhre R, Jhonsborg O, Aakra A, Nes I. F, Inducible bacteriocin production
in Lactobacillus is regulated by differential
expression of the Pln operons and by two antagonizing response regulators, the activity
of which is enhanced upon phosphorylation.
Molecular Microbiology. 2003; 47(2):483–494.
[4] Maldonado A, Ruiz-Barba J. L, JimenezDiaz R., Purification and genetic characterization of plantaricin NC8, a novel cocultureinducible two-peptide bacteriocin from Lactobacillus plantarum NC8. Appl Environ
Microbiol. 2003; 69:383–389.
[5] Kim W.S, Khunajakr N, Ren J, Dunn N.W,
Conservation of the Major Cold Shock Protein in Lactic Acid Bacteria. Current Microbiology. 1998; 37(5):333–336.
[6] Wouters J. A, Rombouts F. M, Kuipers O. P,
de Vos W. M., Abee T., The role of coldshock proteins in low-temperature adaptation of food-related bacteria. Systematic and
Applied Microbiology. 2000 ; 23(2):165–73.
[7] Derzelle S, Hallet B, Ferain T, Delcour J, Hols P. Improved Adaptation to
Cold-Shock, Stationary-Phase, and Freezing Stresses in Lactobacillus plantarum
Overproducing Cold-Shock Proteins. Applied and Environmental microbiology.
2003;4285–4290.
[8] Tai F. H, Foo L. H, Rahim A. R, Loh C.
T, Abdullah P. M, Yoshinobu K. Molecular characterisation of new organisation of
PlnEF and plw loci of bacteriocin genes harbour concomitantly in Lactobacillus plantarum I-UL4. Microb Cell Fact. 2015;
14:89–102.
[9] Remiger A, Ehrmann M. A, Vogel R.
F, Identification of bacteriocin-encoding
genes in lactobacilli by polymerase chain
reaction (PCR). Systematic and Applied Microbiology. 1996; 19(1):28–34.
[10] Sambrook J, Russell D W. Molecular cloning: A laboratory manual. 3rd edition. Cold Spring Harbor Laboratory, Cold
Spring Harbor, NY. 2001.
[11] Nation Center for Biotechnology Information. Blastn suite. 2021. Truy cập
từ: https://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&PAGE_TYPE=BlastSearch&LINK_LOC=blasthome
[Ngày truy cập 11/12/2020].
[12] Muller D. M, Carrasco M. S, Tonarelli G. ¨
G, Simonetta A. C. Characterization and purification of a new bacteriocin with a broad
inhibitory spectrum produced by Lactobacillus plantarum lp 31 strain isolated from
dry-fermented sausage. Journal of Applied Microbiology. 2009; 106(6):2031–2040.
[13] Smaoui S, Elleuch L, Bejar W, KarrayRebai I, Ayadi I, Jaouadi B, Mathieu F,
Chouayekh H, Bejar S, Mellouli L. Inhibition of Fungi and Gram-Negative Bacteria by Bacteriocin BacTN635 Produced by Lactobacillus plantarum sp. TN635. Applied Chemistry and Biotechnology. 2010;
23:1132–1146.
[14] Sáenz Y, Rojo-Bezares B, Navarro L, Díez L, Somalo S, Zarazaga M, Ruiz-Larrea F,
Torres C, Genetic diversity of the plnlocus
among oenological Lactobacillus plantarum
strains. Internation Journal of Microbiology. 2009; 134(3):176–183.
[15] Uyen Q. N, Anh H. T. D, Le M. T. N. Some characteristics of the bacteriocin and
cold shock protein of the strain Lactobacillus plantarum UL485 isolated from chao of
Hue province in Vietnam. BioTechnology: An Indian Journal. 2018; 14(5):171–175.