EFFECTS OF Bacillus amyloliquefaciens D19 CULTURE CONDITIONS ON IN-VITRO ANTIFUNGAL ACTIVITY AGAINST Neoscytalidium dimidiatum
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Published:
Sep 6, 2024
Keywords:
antifungal, Bacillus amyloliquefaciens, culture condition, dragon fruit, Neoscytalidium dimidiatum
Main Article Content
Abstract
The fungus Neoscytalidium dimidiatum is a pathogen that causes diseases in a variety of plants, with a particular preference for dragon fruit. In the search for safe and effective control of N. dimidiatum diseases in dragon fruit, the focus has been directed towards research of biological controls. This study investigates the Bacillus amyloliquefaciens D19 culture conditions for favorable antifungal activity against N dimidiatum. The highest antifungal activity was achieved in culture maintained at 45oC with agitation at 150 rpm for 21 hours in the defined medium comprised of 0.5 g/L MgSO4.7H2O, 3.0 g/L NaH2PO4.2H2O, 3.0 g/L Na2HPO4.12H2O, 10 g/L sucrose, and 10 g/L peptone with initial pH 6.0. Additionally, the antifungal activity of the bacterial culture was found to be stable at temperatures less than 60oC and pH conditions ranging from 7.0 to 9.0. Overall, the findings of this study lay a foundation for future research into the application of biological antagonists in the
control of N. dimidiatum diseases in plants.
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1.
Nguyen NA, Nguyen PT, Hua TC, Nguyen HTD, Pham TV. EFFECTS OF Bacillus amyloliquefaciens D19 CULTURE CONDITIONS ON IN-VITRO ANTIFUNGAL ACTIVITY AGAINST Neoscytalidium dimidiatum. journal [Internet]. 6Sep.2024 [cited 18Oct.2024];14(3). Available from: https://journal.tvu.edu.vn/index.php/journal/article/view/4384
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References
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amyloliquefaciens D19 against Colletotrichum siamense [Khảo sát đặc điểm đối kháng Colletotrichum
siamense của chủng vi khuẩn Bacillus amyloliquefaciens D19]. Journal of Science and Technology-IUH
[Tạp chí Khoa học và Công nghệ-IUH]. 2023;65(05):
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iturin a from Bacillus against Aspergillus
niger. Journal of Fungi. 2024;10(3): 172.
https://doi.org/10.3390/jof10030172.
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Bacillus amyloliquefaciens M13-RW0 from Soil
and Evaluation of its Antifungal Activity. Current
Drug Discovery Technologies. 2023;20(4): 48–54.
https://doi.org/10.2174/1570163820666230419090347.
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wilt in watermelon by Bacillus amyloliquefaciens DHA55 through extracellular production of antifungal lipopeptides. Journal of Fungi. 2023;9(3): 336.
https://doi.org/10.3390/jof9030336.
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https://doi.org/10.1007/s13205-013-0134-4.
[21] Song L, Jiang N, Wei S, Lan Z, Pan L.
Isolation, screening, and identification of actinomycetes with antifungal and enzyme activity
assays against Colletotrichum dematium of Sarcandra glabra. Mycobiology. 2020;48(1): 37–43.
https://doi.org/10.1080/12298093.2020.1716604.
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Isolation and biochemical characterisation of a
bacteriocin-like substance produced by Bacillus
amyloliquefaciens An6. Journal of Global
Antimicrobial Resistance. 2015;3(4): 255–261.
https://doi.org/10.1016/j.jgar.2015.07.001.
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TDH, Nguyen LHH, Tran TTT, et al. Isolation,
identification and characterization of bacterial
antagonists of the dragon fruit fungal pathogen
Neoscytalidium dimidiatum. Journal of Science
and Technology-IUH. 2020;44(02): 76–85.
https://doi.org/10.46242/jst-iuh.v44i02.1036.
[24] Nguyen NA, Trinh TKN, Nguyen TDH, Pham TV.
Effects of culture conditions on Bacillus licheniformis
KN12 antifungal activity against Fusarium oxysporum
and Fusarium equiseti. In: Hội nghị Nấm học toàn
quốc lần thứ 4; 2022; Cần Thơ: Nhà xuất bản
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on Bacillus licheniformis KN12 antifungal activity
against Fusarium oxysporum and Fusarium equiseti.
In: The 4th National Conference on Mycology; 2022;
Can Tho: Publishing House for Science and Technology].
[25] Vu KD, Ho TT, Nguyen TN. Levan production by
Bacillus subtilis natto D strain [Nghiên cứu sản xuất
levan từ Bacillus subtilis natto D]. Journal of Forestry
Science and Technology [Tạp chí Khoa học và Công
nghệ Lâm nghiệp]. 2017;20(10): 11–18.
[26] Yew SM, Chan CL, Lee KW, Na SL, Tan R,
Hoh CC, et al. A five-year survey of dematiaceous
fungi in a tropical hospital reveals potential opportunistic species. PLoS one. 2014;9(8): e104352.
https://doi.org/10.1371/journal.pone.0104352.
[27] James JE, Santhanam J, Lee MC, Wong CX, Sabaratnam P, Yusoff H, et al. In vitro antifungal susceptibility of Neoscytalidium dimidiatum clinical isolates
from Malaysia. Mycopathologia. 2017;182(3-4): 305–
313.
[28] Futatsuya T, Ogawa A, Anzawa K, Mochizuki
T, Shimizu A. First isolation of Neoscytalidium
dimidiatum from human dermatomycosis in Japan.
Medical Mycology Journal. 2022;63(3): 71–75.
https://doi.org/10.3314/mmj.22-00005.
[29] Dang Y, Zhao F, Liu X, Fan X, Huang R, Gao W,
et al. Enhanced production of antifungal lipopeptide
iturin A by Bacillus amyloliquefaciens LL3 through
metabolic engineering and culture conditions optimization. Microbial Cell Factories. 2019;18(1): 68.
https://doi.org/10.1186/s12934-019-1121-1.
[30] Chen W, Wu Z, He Y. Isolation, purification, and
identification of antifungal protein produced
by Bacillus subtilis SL-44 and anti-fungal
resistance in apple. Environmental Science and
Pollution Research. 2023;30(22): 62080–62093.
https://doi.org/10.1007/s11356-023-26158-3.
[31] Baptista JP, Teixeira GM, de Jesus MLA, Bertê R,
Higashi A, Mosela M, et al. Antifungal activity
and genomic characterization of the biocontrol agent
Bacillus velezensis CMRP 4489. Scientific Reports.
2022;12(1): 17401. https://doi.org/10.1038/s41598-
022-22380-0.
[32] Mosquera S, González-Jaramillo LM, Orduz S,
Villegas-Escobar V. Multiple response optimization
of Bacillus subtilis EA-CB0015 culture and identification of antifungal metabolites. Biocatalysis and
Agricultural Biotechnology. 2014;3(4): 378–385.
[33] Khan N, Martínez-Hidalgo P, Ice TA, Maymon
M, Humm EA, Nejat N, et al. Antifungal activity of Bacillus species against Fusarium and analysis of the potential mechanisms used in biocontrol. Frontiers in Microbiology. 2018;9: 1–12.
https://doi.org/10.3389/fmicb.2018.02363.
[34] Sidorova TM, Asaturova AM, Homyak AI,
Zhevnova NA, Shternshis MV, Tomashevich
NS. Optimization of laboratory cultivation
conditions for the synthesis of antifungal metabolites
by Bacillus subtilis strains. Saudi Journal of
Biological Sciences. 2020;27(7): 1879–1885.
https://doi.org/10.1016/j.sjbs.2020.05.002.
[35] Zhang W, Wei L, Xu R, Lin G, Xin H, Lv Z, et
al. Evaluation of the antibacterial material production in the fermentation of Bacillus amyloliquefaciens-9 from whitespotted bamboo shark (Chiloscyllium plagiosum). Marine Drugs. 2020;18(2): 119.
https://doi.org/10.3390/md18020119.
[36] Zhao X, Han Y, Tan XQ, Wang J, Zhou ZJ. Optimization of antifungal lipopeptide production from
Bacillus sp. BH072 by response surface methodology. Journal of Microbiology. 2014;52: 324–332.
https://doi.org/10.1007/s12275-014-3354-3.
[37] Yoshida S, Hiradate S, Tsukamoto T, Hatakeda K,
Shirata A. Antimicrobial activity of culture fltrate
of Bacillus amyloliquefaciens RC-2 isolated from
mulberry leaves. Phytopathology. 2001;91: 181–187.
https://doi.org/10.1094/PHYTO.2001.91.2.181.
N. Scytalidium and scytalidiosis: what’s new in 2012?
Journal of Medical Mycology. 2013;23(1): 40–46.
https://doi.org/10.1016/j.mycmed.2013.01.002.
[2] Elad D, Morick D, David D, Scheinin A,
Yamin G, Blum S, et al. Pulmonary fungal
infection caused by Neoscytalidium dimidiatum
in a Risso’s dolphin (Grampus griseus).
Medical Mycology. 2011;49(4): 424–426.
https://doi.org/10.3109/13693786.2010.533392.
[3] Xu M, Peng Y, Qi Z, Yan Z, Yang L, He MD, et al. Identification of Neoscytalidium dimidiatum causing canker disease of pitaya in Hainan,
China. Australasian Plant Pathology. 2018;47: 547–
553. https://doi.org/10.1007/s13313-018-0588-2.
[4] Turk ¨ olmez S¸, Dervis¸ S, C¸ iftc¸i O, Serc¸e C¸ U, Dikil- ¨
itas M. New disease caused by Neoscytalidium
dimidiatum devastates tomatoes (Solanum lycopersicum) in Turkey. Crop Protection. 2019;118: 21–30.
https://doi.org/10.1016/j.cropro.2018.12.004.
[5] Corkley I, Fraaije B, Hawkins N. Fungicide resistance
management: Maximizing the effective life of plant
protection products. Plant Pathology. 2022;71(1):
150–169. https://doi.org/10.1111/ppa.13467.
[6] Su YT, Liu C, Long Z, Ren H, Guo XH. Improved production of spores and bioactive metabolites from Bacillus amyloliquefaciens in solid-state
fermentation by a rapid optimization process. Probiotics and Antimicrobial Proteins. 2019;11: 921–930.
https://doi.org/10.1007/s12602-018-9474-z.
[7] Abd-Elhalim BT, Gamal RF, El-Sayed SM, AbuHussien SH. Optimizing alpha-amylase from Bacillus
amyloliquefaciens on bread waste for effective industrial wastewater treatment and textile desizing through
response surface methodology. Scientific Reports.
2023;13(1): 19216. https://doi.org/10.1038/s41598-
023-46384-6.
[8] Lambré C, Barat Baviera JM, Bolognesi C,
Cocconcelli PS, Crebelli R, Gott DM, et al.
Safety evaluation of the food enzyme bacillolysin from the non-genetically modified Bacillus amyloliquefaciens strain NZYM-NB. European
Food Safety Authority Journal. 2024;22(2): 1–13.
https://doi.org/10.2903/j.efsa.2023.8392.
[9] WoldemariamYohannes K, Wan Z, Yu Q, Li H,
Wei X, Liu Y, et al. Prebiotic, probiotic, antimicrobial, and functional food applications of
Bacillus amyloliquefaciens. Journal of Agricultural
and Food Chemistry. 2020;68(50): 14709–14727.
https://doi.org/10.1021/acs.jafc.0c06396.
[10] Wang B, Zhou Y, Tang L, Zeng Z, Gong L, Wu
Y, et al. Effects of Bacillus amyloliquefaciens instead of antibiotics on growth performance, intestinal health, and intestinal microbiota of broilers.
Frontiers in Veterinary Science. 2021;8: 269368.
https://doi.org/10.3389/fvets.2021.679368.
[11] Xue M, Wu Y, Hong Y, Meng Y, Xu C, Jiang N,
et al. Effects of dietary Bacillus amyloliquefaciens
on the growth, immune responses, intestinal microbiota composition and disease resistance of yellow
catfish, Pelteobagrus fulvidraco. Frontiers in Cellular and Infection Microbiology. 2022;12: 1047351.
https://doi.org/10.3389/fcimb.2022.1047351.
[12] Chen L, Liu Y, Wu G, Veronican Njeri K, Shen
Q, Zhang N, et al. Induced maize salt tolerance by
rhizosphere inoculation of Bacillus amyloliquefaciens
SQR9. Physiologia Plantarum. 2016;158(1): 34–44.
https://doi.org/10.1111/ppl.12441.
[13] Tiwari S, Prasad V, Chauhan PS, Lata C. Bacillus
amyloliquefaciens confers tolerance to various abiotic
stresses and modulates plant response to phytohormones through osmoprotection and gene expression
regulation in rice. Frontiers in Plant Science. 2017;8:
1–13. https://doi.org/10.3389/fpls.2017.01510.
[14] Bisht N, Mishra SK, Chauhan PS. Bacillus amyloliquefaciens inoculation alters physiology of rice
(Oryza sativa L. var. IR-36) through modulating carbohydrate metabolism to mitigate stress induced by nutrient starvation. International Journal
of Biological Macromolecules. 2020;143: 937–951.
https://doi.org/10.1016/j.ijbiomac.2019.09.154.
[15] Nguyen TDH, Pham DT, Nguyen HV, Pham TV,
Nguyen NA. Antagonistic characteristics of Bacillus
amyloliquefaciens D19 against Colletotrichum siamense [Khảo sát đặc điểm đối kháng Colletotrichum
siamense của chủng vi khuẩn Bacillus amyloliquefaciens D19]. Journal of Science and Technology-IUH
[Tạp chí Khoa học và Công nghệ-IUH]. 2023;65(05):
69–80. https://doi.org/10.46242/jstiuh.v65i05.4962.
[16] Li X, Zhang Y, Wei Z, Guan Z, Cai Y, Liao
X. Antifungal activity of isolated Bacillus amyloliquefaciens SYBC H47 for the biocontrol of
peach gummosis. PLoS One. 2016;11(9): e0162125.
https://doi.org/10.1371/journal.pone.0162125.
[17] Wang S, Xu M, Han Y, Zhou Z. Exploring
mechanisms of antifungal lipopeptide
iturin a from Bacillus against Aspergillus
niger. Journal of Fungi. 2024;10(3): 172.
https://doi.org/10.3390/jof10030172.
[18] Naghdifar S, Madani M, Shakib P. Isolation of
Bacillus amyloliquefaciens M13-RW0 from Soil
and Evaluation of its Antifungal Activity. Current
Drug Discovery Technologies. 2023;20(4): 48–54.
https://doi.org/10.2174/1570163820666230419090347.
[19] Al-Mutar DMK, Alzawar NSA, Noman M, Azizullah, Li D, Song F. Suppression of Fusarium
wilt in watermelon by Bacillus amyloliquefaciens DHA55 through extracellular production of antifungal lipopeptides. Journal of Fungi. 2023;9(3): 336.
https://doi.org/10.3390/jof9030336.
[20] Ashwini N, Srividya S. Potentiality of Bacillus subtilis as biocontrol agent for management of anthracnose disease of chilli caused by Colletotrichum
gloeosporioides OGC1. 3 Biotech. 2014;4(2): 127–36.
https://doi.org/10.1007/s13205-013-0134-4.
[21] Song L, Jiang N, Wei S, Lan Z, Pan L.
Isolation, screening, and identification of actinomycetes with antifungal and enzyme activity
assays against Colletotrichum dematium of Sarcandra glabra. Mycobiology. 2020;48(1): 37–43.
https://doi.org/10.1080/12298093.2020.1716604.
[22] Ayed HB, Maalej H, Hmidet N, Nasri M.
Isolation and biochemical characterisation of a
bacteriocin-like substance produced by Bacillus
amyloliquefaciens An6. Journal of Global
Antimicrobial Resistance. 2015;3(4): 255–261.
https://doi.org/10.1016/j.jgar.2015.07.001.
[23] Nguyen NA, Hua HMT, Ho NHY, Nguyen
TDH, Nguyen LHH, Tran TTT, et al. Isolation,
identification and characterization of bacterial
antagonists of the dragon fruit fungal pathogen
Neoscytalidium dimidiatum. Journal of Science
and Technology-IUH. 2020;44(02): 76–85.
https://doi.org/10.46242/jst-iuh.v44i02.1036.
[24] Nguyen NA, Trinh TKN, Nguyen TDH, Pham TV.
Effects of culture conditions on Bacillus licheniformis
KN12 antifungal activity against Fusarium oxysporum
and Fusarium equiseti. In: Hội nghị Nấm học toàn
quốc lần thứ 4; 2022; Cần Thơ: Nhà xuất bản
Khoa học và Công nghệ. [Nguyen NA, Trinh TKN,
Nguyen TDH, Pham TV. Effects of culture conditions
on Bacillus licheniformis KN12 antifungal activity
against Fusarium oxysporum and Fusarium equiseti.
In: The 4th National Conference on Mycology; 2022;
Can Tho: Publishing House for Science and Technology].
[25] Vu KD, Ho TT, Nguyen TN. Levan production by
Bacillus subtilis natto D strain [Nghiên cứu sản xuất
levan từ Bacillus subtilis natto D]. Journal of Forestry
Science and Technology [Tạp chí Khoa học và Công
nghệ Lâm nghiệp]. 2017;20(10): 11–18.
[26] Yew SM, Chan CL, Lee KW, Na SL, Tan R,
Hoh CC, et al. A five-year survey of dematiaceous
fungi in a tropical hospital reveals potential opportunistic species. PLoS one. 2014;9(8): e104352.
https://doi.org/10.1371/journal.pone.0104352.
[27] James JE, Santhanam J, Lee MC, Wong CX, Sabaratnam P, Yusoff H, et al. In vitro antifungal susceptibility of Neoscytalidium dimidiatum clinical isolates
from Malaysia. Mycopathologia. 2017;182(3-4): 305–
313.
[28] Futatsuya T, Ogawa A, Anzawa K, Mochizuki
T, Shimizu A. First isolation of Neoscytalidium
dimidiatum from human dermatomycosis in Japan.
Medical Mycology Journal. 2022;63(3): 71–75.
https://doi.org/10.3314/mmj.22-00005.
[29] Dang Y, Zhao F, Liu X, Fan X, Huang R, Gao W,
et al. Enhanced production of antifungal lipopeptide
iturin A by Bacillus amyloliquefaciens LL3 through
metabolic engineering and culture conditions optimization. Microbial Cell Factories. 2019;18(1): 68.
https://doi.org/10.1186/s12934-019-1121-1.
[30] Chen W, Wu Z, He Y. Isolation, purification, and
identification of antifungal protein produced
by Bacillus subtilis SL-44 and anti-fungal
resistance in apple. Environmental Science and
Pollution Research. 2023;30(22): 62080–62093.
https://doi.org/10.1007/s11356-023-26158-3.
[31] Baptista JP, Teixeira GM, de Jesus MLA, Bertê R,
Higashi A, Mosela M, et al. Antifungal activity
and genomic characterization of the biocontrol agent
Bacillus velezensis CMRP 4489. Scientific Reports.
2022;12(1): 17401. https://doi.org/10.1038/s41598-
022-22380-0.
[32] Mosquera S, González-Jaramillo LM, Orduz S,
Villegas-Escobar V. Multiple response optimization
of Bacillus subtilis EA-CB0015 culture and identification of antifungal metabolites. Biocatalysis and
Agricultural Biotechnology. 2014;3(4): 378–385.
[33] Khan N, Martínez-Hidalgo P, Ice TA, Maymon
M, Humm EA, Nejat N, et al. Antifungal activity of Bacillus species against Fusarium and analysis of the potential mechanisms used in biocontrol. Frontiers in Microbiology. 2018;9: 1–12.
https://doi.org/10.3389/fmicb.2018.02363.
[34] Sidorova TM, Asaturova AM, Homyak AI,
Zhevnova NA, Shternshis MV, Tomashevich
NS. Optimization of laboratory cultivation
conditions for the synthesis of antifungal metabolites
by Bacillus subtilis strains. Saudi Journal of
Biological Sciences. 2020;27(7): 1879–1885.
https://doi.org/10.1016/j.sjbs.2020.05.002.
[35] Zhang W, Wei L, Xu R, Lin G, Xin H, Lv Z, et
al. Evaluation of the antibacterial material production in the fermentation of Bacillus amyloliquefaciens-9 from whitespotted bamboo shark (Chiloscyllium plagiosum). Marine Drugs. 2020;18(2): 119.
https://doi.org/10.3390/md18020119.
[36] Zhao X, Han Y, Tan XQ, Wang J, Zhou ZJ. Optimization of antifungal lipopeptide production from
Bacillus sp. BH072 by response surface methodology. Journal of Microbiology. 2014;52: 324–332.
https://doi.org/10.1007/s12275-014-3354-3.
[37] Yoshida S, Hiradate S, Tsukamoto T, Hatakeda K,
Shirata A. Antimicrobial activity of culture fltrate
of Bacillus amyloliquefaciens RC-2 isolated from
mulberry leaves. Phytopathology. 2001;91: 181–187.
https://doi.org/10.1094/PHYTO.2001.91.2.181.