RAPID DETECTION OF Vibrio parahaemolyticus USING POLYDOPAMINE MEMBRANES IN COMBINATION WITH LAMP

Abstract

 This study was conducted from September 2025 to April 2026 at Tra Vinh University, Vietnam, with the aim of developing a simple detection strategy for screening Vibrio parahaemolyticus using polydopamine membranes and loop-mediated isothermal amplification. Polydopamine membranes were synthesized by coating Whatman filter paper with dopamine and used for nucleic acid extraction. The extracted DNA was then amplified using loopmediated isothermal amplification at 65oC for 25 minutes. Results showed that an incubation time of 20 minutes with a suitable A260/A280 ratio was selected for downstream analysis, including amplification and detection. The loop-mediated isothermal amplification result was directly observed due to the color change from red (negative) to yellow (positive), indicating samples containing the target Vibrio parahaemolyticus. This detection method opens potential applications for rapid bacterial detection in low-resource areas.