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In the schrimp-farming process at Vietnam has used antibiotic mostly, this leads status of antibiotic resistant bacteria and product do not qualified to the market. Bacteria, especially actinobacteria, had resistantant ability to human pathogenic bacteria in water and they have an important role in sustainable aquaculture. This study aimed to isolate and select good actinobacterial strains against human pathogenic bacteria, from 8 samples of schrimp-pond water at 3 sites Ngu Lac,
Phuoc Tri and Long Toan of Duyen Hai district, Tra Vinh province on Gause-1 agar medium. Fiftythree actinobacterial isolates were isolated and 12 isolates were identifed as resistant to Bacillus cereus and 8 isolates were identifed as resistant to Escherichia coli by well-diffusion method. There were 7 isolates had good resistance to select for PCR technique and sequencing and the result were determined 7 these strains: NL1-1.9, NL1-18a, NL2-2,1b1, NL2-2.2, PT1-1.7a, PT2-2.8a,
LT1-1.1 belonged to three genere: Streptomyces, Nocardioides, Glutamicibacter.
from three Hawaiin marine sponges. Microbiol Res.
 Sambrook J, Fritsch EP, and Manlatis T, 1989. In
vitro amplification of DNA by the polymerase chain
reaction. In: Molecular cloning. A Laboratory Manual 2nd edn. New York: Cold Spring Habor Laboratory Press. 14: 2 - 35.
 Galindo AB, 2004. Lactobacillus plantarum 44A as
a live feed supperlement for freshwater fish. Ph.D
Thesis. pp: 1-131.
 Sheng Q, Wen-Jun L, Syed GD, and Wael NH, 2003.
New primers for the class Actinobacteria: Application to marine and terrestrial environments. Applied
and Environmental Microbiology. 75(19): 6176-86.
 Pandey B, Ghimire P, and Agrawal VP, 2004. Studies on the antibacterial activity of the actinomycetes
isolated from the Khumbu region of Nepal. Acad Sci
 Lechevalier H, 1982. The development of applied
microbiology at Rutgers, The State University of New
 Tanaka Y and Omura O, 1990. Metabolisms and
products of actinomycetes – An Introduction. Actinomycetologica 4:13–14
 Takaizawa M, Colwell W and Hill RT, 1993. Isolation
and diversity of actinomycetes in the Chesapeake
Bay. Appl Environ Microbiol. 59:997–1002
 Goodfellow M and O’Donnell AG, 1989. Roots of
Bacterial Systematic. In: The Handbook of new bacterial systematic. Academic Press, London. pp 3–54
 Goodfellow M and Haynes JA, 1984. Actinomycetes
in marine sediments. In: Biological, biochemical and
biomedical aspects of actinem (Ortiz-Ortiz I, Bojalil
LF & Yakoleff V eds). Academic Press, London. pp
 Dharmaraj S, 2010. Marine Streptomyces as a
novel source of bioactive substances. Review.
World J Microbiol Biotechnol. 26:2123-213 DOI
 Sivakumar K, Kumar M.S, Thangaradjou T, and Kannan L, 2007. Research on marine actinobacteria in
India. J. Microbiol. 47:186–196
 Colwell RR and Hill RT, 1992. Microbial diversity.
In: Diversity of Oceanic Life: An Evaluative Review
(Peterson MNA ed). The Centre for Strategic and
International Studies, Washington, DC. pp 100-106
 Adinarayana G, Venkateshan MR, Bapiraju VV, Sujatha P, Premkumar J, Ellaiah P, and Zeeck A, 2006.
Cytotoxic com-pounds from the marine actinobacterium. Bioorg Khim. 32: 328-334
 Meiying Z and Zhicheng Z, 1998. Identification of
marine actinomycetes S-216 strain and its biosynthetic conditions of antifungal antibiotic. J Xiamen
Univ Nat Sci. 37:109-114