INVESTIGATING FACTORS AFFECTING PRODIGIOSIN PRODUCTION FROM Serratia marcescens SR3 BY SUBMERGED FERMENTATION
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Published:
Dec 30, 2020
Keywords:
medium, pH, prodigiosin, Serratia marcescens, time of fermentation
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Abstract
Prodigiosin is a red pigment and widely applied as antimicrobial, antifungal, anti-malarial, anti-cancer, ,...
Prodigiosin is isolated from bacteria, such as Serratia marcescens, Hahella chejuensis, Vibrio psychroerythrus and Streptomyces coelicolor,. . . S. marcescens is the major producer of prodigiosin. The production of prodigiosin has been shown to be influenced by many factors, such as species type and environmental factors, including media composition, temperature, pH and incubation time,... This study was conducted to analyze the effect of various factors on prodigiosin production process of S. marcescens SR3 by submerged fermentation method. Prodigiosin content was determined by the spectrophotometer method. The results showed that
S. marcescens SR3 could produce prodigiosin in LB medium, initial bacterial volume (7%, v/v), carbon source (lactose 1 g/L), nitrogen source (yeast extract 5 g/L), pH = 8, fermentation time (96 hours), and prodigiosin content reached 527.28 Unit/cell. The results of the study showed that S. marcescens SR3 bacteria is a potential source of prodigiosin for research and applications.
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1.
Nguyen T, Bang L. INVESTIGATING FACTORS AFFECTING PRODIGIOSIN PRODUCTION FROM Serratia marcescens SR3 BY SUBMERGED FERMENTATION. journal [Internet]. 30Dec.2020 [cited 20Apr.2024];10(40):131-4. Available from: https://journal.tvu.edu.vn/index.php/journal/article/view/624
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References
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K, Yamasaki M. Immunomodulating properties of prodigiosin 25-C, an antibiotic
which preferentially suppresses of cytotoxic T cells. Journal of Antibiotics. 1992; 45:1295–1302.
[2] Wang SL, Wang CY, Yen YH. Enhanced
production of insecticidal prodigiosin from
Serratia marcescens TKU011 in media
containing squid pen. Process Biochem. 2011;47:1684–1690.
[3] Siva R, Subha K, Bhakta D. Characterization and enhanced production of prodigiosin
from the spoiled coconut. Applied Biochemistry and Biotechnology. 2012;166:187–196.
[4] Helvia W, Casullo de A, Fukushima K,
Galba M, Campos T. Prodigiosin Production by Serratia marcescens UCP 1549Using Renewable-Resources as a Low Cost Substrate. Molecules. 2010; 15:6931–6940; DOI:10.3390/molecules15106931.
[5] Antony V, Samrot L, Chandana K,
Senthilkumar PG. Optimized Production of
Prodigiosin from Serratia marcescens SU-
10 Grown as Batch Culture and Evaluation
of Bioactivity of Produced Prodigiosin.
International Journal of Medicobiological. 2011; 1(3):145–150.
[6] Chandni G, Sourav B, Arijit D. Assessment
of Process Parameters Influencing the Enhanced Production of Prodigiosin from Serratia marcescens and Evaluation of Its Antimicrobial, Antioxidant and Dyeing Potentials. Malaysian Journal of Microbiology.
2012;8(2):116– 122.
[7] Shahitha S, Poornima K. Enhanced production of prodigiosin production in Serratia
marcescens. Journal of Applied Pharmaceutical Science. 2012; 2(8):138–140.
[8] San LW, Yue HY, Tzu WL, Shin YC,
Chia HC. Enhanced production of insecticidal prodigiosin from Serratia marcescens
TKU011 in media containing squid pen.
Process Biochemistry. 2012; 47(11):1684–1690.
[9] Darshan N, Manonmani HK. Prodigiosin
and its potential applications. Journal
of Food Science and Technology. 2016;
52:5393–5407. DOI: 10.1007/s13197-015-1740-4.
[10] Anuradha V.G, Anandkumar N, Muthukumaran G. A novel medium for the enhanced cell growth and production of prodigiosin from Serratia marcescens isolated
from soil. BMC Microbiol. 2004; 4:11–18.
[11] Kurbanoglu EB, Ozdal OG, Algur OF. Enhanced production of prodigiosin by Serratia marcescens MO-1 using ram horn peptone. Braz J Microbiol. 2015;46:631–637.
[12] Ahmad WA, Wan Ahmad WY, Zakaria
ZA, Yusof NZ. Application of Bacterial Pigments as Colorant; 2012. DOI:
10.1007/978-3-642-24520-6.
[13] Buckland B, Gbewonyo K., Hallada T.,
Kaplan L., Masurekar P. Production of
lovastatin, an inhibitor of cholesterol
accumulation in humans. In: Novel
Microbial Products for Medicine and
Agriculture, A.L.Demain, G.A.Somkuti,
JC.Hunter-Cevera and H.W.Rossmoore, eds. Elsevier. 1989; p.161–169.
[14] Giri AV, Anandkumar N, Muthukumaran G. A novel medium for the enhanced cell
growth and production of prodigiosin from
Serratia marcescens isolated from soil. BMC Microbiol. 2004; 4:11–18.
[15] Sole M, Francia A, Rius N. The role of
pH in the glucose effect on prodigiosin
production by non-proliferating cells of S.
marcescens. Lett Appl Microbiol. 1997; 25:81–84.
[16] Vijay Singh Gondil, Mohammad Asif, Tek
Chand Bhalla. Optimization of physicochemical parameters influencing the production of prodigiosin from Serratia nematodiphila RL2 and exploring its antibacterial
activity. Biotech. 2017; 7:338–345.
[17] Furstner A. Chemistry and biology of
roseophilin and the prodigiosin alkaloids:
a survey of the last 2500 years. Angewandte Chemie International Edition. 2003; 42:3582–3603.
[18] Sundaramoorthy N, Yogesh P, Dhandapani
R. Production of prodigiosin from Serratia
marcescens isolated from soil. Indian Journal of Science and Technology. 2009; 2:32–34.
[19] Gargallo D, Loren JG, Guinea J, Vina
M. Glucose-6-phosphate dehydrogenase alloenzymes and their relationship to pigmentation in Serratia marcescens. Appl Environ
Microbiol. 1987; 53:1983–1986.
[20] Hejazi A, Falkiner FR. Serratia marcescens.
Journal of Medical Microbiology. 1997; 46:903–912.
[21] Manzoni M, M Rollini. Biosynthesis and
Biotechnological production of statins by
filamentous fungi and application of these
cholesterol-lowering drugs. Applied Microbiology and Biotechnology. 2002; 58:555–564.
[22] Andrade RFS, Luna JM, Rufino RD, Costa
Albuquerque CD, Sarubbo LA, Takaki GM.
Surface active agent produced by Candida
lipolytica using cassava flour wastewater as
substrate. In: Current research topics in applied microbiology and microbial biotechnology. Singapore: World Scientific Publishing Co. Pte. Ltd. 2009; p.751–756.
[23] Suryawanshi RK, Patil CD, Borase HP,
Narkhede CP, Shinde L, Patil SV. Studies
on production and biological potential of
prodigiosin by Serratia marcescens. Appl
Biochem Biotechnol. 2014; 173:1209–1221.
[24] Fuqua C, Winans SC, Greenberg EP. Census and consensus in bacterial ecosystem:
the LuxR-LuxI family of quorum-sensing
transcriptional regulators. Annual Review of
Microbiology. 1996; 50:727–751.
[25] Haddix PL, Werner TF. Spectrophotometric assay of gene expression: Serratia
marcescens pigmentation. Biosciene. 2000; 26:3–13.
[26] Sadia J, Meraj M, Mahmood S, Hameed
A, Naz F, Hassan S, Irfan R. Biosynthesis
of lovastatin using agro-industrial wastes
as carrier substrates. Tropical Journal of
Pharmaceutical Research February. 2017;
16(2):263–269.
[27] Srimathi R, Priya R, Malarvizhi A.
Isolation, Identification, Optimization
of Prodigiosin Pigment Produced by
Serratia marcescens and its Applications.
International Journal of Latest Engineering
and Management Research. 2017; 2(9):11–21.
K, Yamasaki M. Immunomodulating properties of prodigiosin 25-C, an antibiotic
which preferentially suppresses of cytotoxic T cells. Journal of Antibiotics. 1992; 45:1295–1302.
[2] Wang SL, Wang CY, Yen YH. Enhanced
production of insecticidal prodigiosin from
Serratia marcescens TKU011 in media
containing squid pen. Process Biochem. 2011;47:1684–1690.
[3] Siva R, Subha K, Bhakta D. Characterization and enhanced production of prodigiosin
from the spoiled coconut. Applied Biochemistry and Biotechnology. 2012;166:187–196.
[4] Helvia W, Casullo de A, Fukushima K,
Galba M, Campos T. Prodigiosin Production by Serratia marcescens UCP 1549Using Renewable-Resources as a Low Cost Substrate. Molecules. 2010; 15:6931–6940; DOI:10.3390/molecules15106931.
[5] Antony V, Samrot L, Chandana K,
Senthilkumar PG. Optimized Production of
Prodigiosin from Serratia marcescens SU-
10 Grown as Batch Culture and Evaluation
of Bioactivity of Produced Prodigiosin.
International Journal of Medicobiological. 2011; 1(3):145–150.
[6] Chandni G, Sourav B, Arijit D. Assessment
of Process Parameters Influencing the Enhanced Production of Prodigiosin from Serratia marcescens and Evaluation of Its Antimicrobial, Antioxidant and Dyeing Potentials. Malaysian Journal of Microbiology.
2012;8(2):116– 122.
[7] Shahitha S, Poornima K. Enhanced production of prodigiosin production in Serratia
marcescens. Journal of Applied Pharmaceutical Science. 2012; 2(8):138–140.
[8] San LW, Yue HY, Tzu WL, Shin YC,
Chia HC. Enhanced production of insecticidal prodigiosin from Serratia marcescens
TKU011 in media containing squid pen.
Process Biochemistry. 2012; 47(11):1684–1690.
[9] Darshan N, Manonmani HK. Prodigiosin
and its potential applications. Journal
of Food Science and Technology. 2016;
52:5393–5407. DOI: 10.1007/s13197-015-1740-4.
[10] Anuradha V.G, Anandkumar N, Muthukumaran G. A novel medium for the enhanced cell growth and production of prodigiosin from Serratia marcescens isolated
from soil. BMC Microbiol. 2004; 4:11–18.
[11] Kurbanoglu EB, Ozdal OG, Algur OF. Enhanced production of prodigiosin by Serratia marcescens MO-1 using ram horn peptone. Braz J Microbiol. 2015;46:631–637.
[12] Ahmad WA, Wan Ahmad WY, Zakaria
ZA, Yusof NZ. Application of Bacterial Pigments as Colorant; 2012. DOI:
10.1007/978-3-642-24520-6.
[13] Buckland B, Gbewonyo K., Hallada T.,
Kaplan L., Masurekar P. Production of
lovastatin, an inhibitor of cholesterol
accumulation in humans. In: Novel
Microbial Products for Medicine and
Agriculture, A.L.Demain, G.A.Somkuti,
JC.Hunter-Cevera and H.W.Rossmoore, eds. Elsevier. 1989; p.161–169.
[14] Giri AV, Anandkumar N, Muthukumaran G. A novel medium for the enhanced cell
growth and production of prodigiosin from
Serratia marcescens isolated from soil. BMC Microbiol. 2004; 4:11–18.
[15] Sole M, Francia A, Rius N. The role of
pH in the glucose effect on prodigiosin
production by non-proliferating cells of S.
marcescens. Lett Appl Microbiol. 1997; 25:81–84.
[16] Vijay Singh Gondil, Mohammad Asif, Tek
Chand Bhalla. Optimization of physicochemical parameters influencing the production of prodigiosin from Serratia nematodiphila RL2 and exploring its antibacterial
activity. Biotech. 2017; 7:338–345.
[17] Furstner A. Chemistry and biology of
roseophilin and the prodigiosin alkaloids:
a survey of the last 2500 years. Angewandte Chemie International Edition. 2003; 42:3582–3603.
[18] Sundaramoorthy N, Yogesh P, Dhandapani
R. Production of prodigiosin from Serratia
marcescens isolated from soil. Indian Journal of Science and Technology. 2009; 2:32–34.
[19] Gargallo D, Loren JG, Guinea J, Vina
M. Glucose-6-phosphate dehydrogenase alloenzymes and their relationship to pigmentation in Serratia marcescens. Appl Environ
Microbiol. 1987; 53:1983–1986.
[20] Hejazi A, Falkiner FR. Serratia marcescens.
Journal of Medical Microbiology. 1997; 46:903–912.
[21] Manzoni M, M Rollini. Biosynthesis and
Biotechnological production of statins by
filamentous fungi and application of these
cholesterol-lowering drugs. Applied Microbiology and Biotechnology. 2002; 58:555–564.
[22] Andrade RFS, Luna JM, Rufino RD, Costa
Albuquerque CD, Sarubbo LA, Takaki GM.
Surface active agent produced by Candida
lipolytica using cassava flour wastewater as
substrate. In: Current research topics in applied microbiology and microbial biotechnology. Singapore: World Scientific Publishing Co. Pte. Ltd. 2009; p.751–756.
[23] Suryawanshi RK, Patil CD, Borase HP,
Narkhede CP, Shinde L, Patil SV. Studies
on production and biological potential of
prodigiosin by Serratia marcescens. Appl
Biochem Biotechnol. 2014; 173:1209–1221.
[24] Fuqua C, Winans SC, Greenberg EP. Census and consensus in bacterial ecosystem:
the LuxR-LuxI family of quorum-sensing
transcriptional regulators. Annual Review of
Microbiology. 1996; 50:727–751.
[25] Haddix PL, Werner TF. Spectrophotometric assay of gene expression: Serratia
marcescens pigmentation. Biosciene. 2000; 26:3–13.
[26] Sadia J, Meraj M, Mahmood S, Hameed
A, Naz F, Hassan S, Irfan R. Biosynthesis
of lovastatin using agro-industrial wastes
as carrier substrates. Tropical Journal of
Pharmaceutical Research February. 2017;
16(2):263–269.
[27] Srimathi R, Priya R, Malarvizhi A.
Isolation, Identification, Optimization
of Prodigiosin Pigment Produced by
Serratia marcescens and its Applications.
International Journal of Latest Engineering
and Management Research. 2017; 2(9):11–21.