BIOACTIVE COMPOUNDS ANALYSIS AND ANTIOXIDANT ACTIVITIES OF Tinospora crispa MIERS STEM EXTRACT
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Published:
Dec 30, 2020
Keywords:
antioxidant, flavonoid, phenolic, polysaccharide, Tinospora crispa
Main Article Content
Abstract
The study was conducted to analyze some of the bioactive compounds and the antioxidant capacity of Tinospora crispa stem extract. Tinospora crispa stem extract is a prerequisite for the production of products capable of supporting and treating diseases. Tinospora crispa stem extract is made by immersion method with
solvents (water, ethanol 80o and methanol) and combined with ultrasound. Antioxidant activities were tested using DPPHmethods and phenolic, flavonoid, polysaccharide, tannin content were determined by the spectrophotometer method. The results showed that the moisture content was 61.09% and high extraction efficiency of Tinospora crispa stem were between 3.69% and 6.95% respectively. The extract of Tinospora crispa stem contains the presence of biological compounds such as alkaloids, flavonoids, saponin, steroids, tannins and phenols. The phenolic, flavonoid, polysaccharide and tannin content of Tinospora crispa stem were
318.91 mg gallic acid/g dry; 36.71 mg quercetin/g dry; 10.38 mg GE/g dry and 38.42 mg tannic acid/g dry respectively. Tinospora crispa stem has antioxidant ability by DPPH method with IC50 value of water, ethanol 80o and methanol 113.69 mg/mg; 89.12 mg/mg; 62.19 mg/mg, respectively. Thus, the Tinospora crispa
stem extract had bioactive compounds and antioxidant activities, is a potential source of raw materials for research and applications.
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1.
Nguyen T, Bang L, Nguyen T, Nguyen T. BIOACTIVE COMPOUNDS ANALYSIS AND ANTIOXIDANT ACTIVITIES OF Tinospora crispa MIERS STEM EXTRACT. journal [Internet]. 30Dec.2020 [cited 20Apr.2024];10(40):58-9. Available from: https://journal.tvu.edu.vn/index.php/journal/article/view/617
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References
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DPPH, Super oxide and nitric oxide free
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2011; 1:52–68.
[24] Tatang I, Andayana P, Machwiyyah L, Rabbani HR. The activity of radical scavenging of 2,2-diphenyl-1- pycrilhydrazil (dpph)
by ethanolic extracts of Mengkudu leaves
(Morinda citrifolia L.), Brotowali stem
(Tinospora crispa L.), its water fraction and
its hydrolized fraction. Traditional Medicine
Journal. 2015; 20 (3):140–148.
[25] Hwang SJ, Yoon WB, Lee OH, Cha SJ, Kim
JD. Radical-scavenging-linked antioxidant
activities of extracts from black chokeberry
and blueberry cultivated in Korea. Food
Chem. 2014; 146:71–77.
[26] Zin ZM, Hamid AA, Osman A, Saari N.
2006. Antioxidative activities of chromatographic fractions obtained from root, fruit
and leaf of Mengkudu (Morinda citrifolia
L.). Food Chem. 2006; 94:169–178.
Quignard ELJ, López JA, Almeida MG.
Turnera ulmifolia L. (Turneraceae): Preliminary study of its antioxidant activity. Bioresources Technology. 2006; 97:1387–1391.
[2] Diplock AT. Will the ‘good fairies’ please
proves to us that Vitamin E lessens human
degenerative of diseases. Free Radical Research. 1997; 27:511–532.
[3] Shahidi F. Antioxidants in food and food
antioxidants. Nahrung. 2000; 44:158–163.
[4] Haque AM, Ashraful SM, Mohammad S.
Antimicrobial, cytotoxicity and antioxidant
activity of Tinospora crispa. Journal of
Phar and Biomed Sciences. 2011; 13(13):1–
4.
[5] Yang X, Yan F, Huang S, Fu C. Antioxidant
activities of fractions from longan pericarps.
Food Science and Technology (Campinas).
2014; 34(2):341–345.
[6] Yadav M, Chatterji S, Watal G. Preliminary
phytochemical screening of six medicinal
plants used in traditional medicine. International Journal of Pharmacy and Pharmaceutical Sciences. 2014; 6(5):539–542.
[7] Pieme C, Joseph N, Claude N, Bruno M,
Borgia N, Vicky M, Jacqueline M, Jeanne
N. Syzyguim guineense Extracts Show Antioxidant Activities and Beneficial Activities on Oxidative Stress Induced by Ferric
Chloride in the Liver Homogenate. Antioxidants. 2014; 3(3):618–635.
[8] Yadav RNS, Agarwala M. Phytochemical
analysis of some medicinal plants. Journal
of Phytology. 2011; 3(12):10–14.
[9] Dubois M, Gilles K, Hamilton J, Rebers
P, Smith F. Colorimetric method for determination of sugars and related substances.
Anal Chem.1956; 28:350–356.
[10] Kavitha CCI, Indira G. Quantitative estimation of total phenolic, flavonoids, tannin
and chlorophyll content of leaves of Strobilanthes Kunthiana (Neelakurinji). Journal of Medicinal Plants Studies. 2016;
4(4):282286.
[11] Shekhar TC, Anju G. Antioxidant Activity by DPPH Radical Scavenging Method
of Ageratum conyzoides Linn. Leaves.
American Journal of Ethnomedicine. 2014;
1(4):244–249.
[12] Bandar H, A. Hijazi, H. Rammal, A.
Hachem, Z. Saad, B Badran. Techniques for
the extraction of bioactive compounds from
Lebanese Urtica Dioica. American Journal
of Phytomedicine and Clinical Therapeutics. 2013; 6:507–513.
[13] Harwoko H, Warsinah W. 2020. Phytochemical analysis and evaluation of purified extract of Tinospora crispa stem for in vivo antihyperuricemic effect. Journal of Reports
in Pharmaceutical Sciences. 2020; 9:46–51.
[14] Sensen Chi, Gaimei She, Dan Han,
Weihua Wang, Zhao Liu, and Bin Liu.
Genus Tinospora: Ethnopharmacology,
Phytochemistry, and Pharmacology.
Evidence-Based Complementary and
Alternative Medicine. 2016; Article ID
9232593:32 pages.
[15] Han X, Shen T, Lou H. Dietary polyphenols
and their biological significance. International Journal of Molecular Sciences. 2007;
8:950–988.
[16] Yao LH, Jiang YM, Datta N, Singanusong
R, Liu X, Duan J, Raymont K, Lisle A, Y
Xu. HPLC analyses of flavanols and phenolic acids in the fresh young shoots of
tea (Camellia sinensis) grown in Australia.
Food Chemistry. 2004; 84(2):253–263.
[17] Heida NZ, Jamaludin M, Zainal A. Antioxidant Activity of Methanol Extract
of Tinospora crispa and Tabernaemontana corymbosa. Sains Malaysiana. 2013;
42(6):697–706.
[18] Harleen KS, Bimlesh K, Manoj S, Pardeep
S. A Review of Phytochemistry and Pharmacology of Flavonoids. International Pharmaceutica Sciencia. 2011; 1(1):25–35.
[19] Turkoglu, E.M. Duru, I.K. Mercan and K.
Gezer. Antioxidant and antimicrobial activities of Laetiporus sulphureus (Bull.) Murrill. Food Chemistry. 2007; 101:267–273.
[20] Zulkhairi A, H Bahari, S Isemaail, N Amalina Ismail, Zamree Md Shah, MS Arsyad.
Nutritional Composition, Antioxidant Ability and Flavonoid Content of Tinospora
crispa stem. Advances in Natural and Applied Sciences. 2009; 3(1):88–94.
[21] Nguyễn Văn Bình, Phạm Thị Phương,
Nguyễn Tá Lợi. Nghiên cứu một số yếu tố
ảnh hưởng đến quá trình trích ly hàm lượng
polysaccharide toàn phần trong nấm linh chi
đỏ. Tạp chí Khoa học và Công nghệ Đại học
Thái Nguyên. 2018; 180(04): 3–8.
[22] Kunwar RM, Shrestha KP, Bussmann RW.
Traditional herbal medicine in far-west
Nepal: a pharmacological appraisal. Journal of Ethnobiolology and Ethnomedicine.
2010; 6:35.
[23] Patel RM, Patel NJ. 2011. In vitro antioxidant activity of coumarin compounds by
DPPH, Super oxide and nitric oxide free
radical scavenging methods. Journal of Advanced Pharmacy Education & Research.
2011; 1:52–68.
[24] Tatang I, Andayana P, Machwiyyah L, Rabbani HR. The activity of radical scavenging of 2,2-diphenyl-1- pycrilhydrazil (dpph)
by ethanolic extracts of Mengkudu leaves
(Morinda citrifolia L.), Brotowali stem
(Tinospora crispa L.), its water fraction and
its hydrolized fraction. Traditional Medicine
Journal. 2015; 20 (3):140–148.
[25] Hwang SJ, Yoon WB, Lee OH, Cha SJ, Kim
JD. Radical-scavenging-linked antioxidant
activities of extracts from black chokeberry
and blueberry cultivated in Korea. Food
Chem. 2014; 146:71–77.
[26] Zin ZM, Hamid AA, Osman A, Saari N.
2006. Antioxidative activities of chromatographic fractions obtained from root, fruit
and leaf of Mengkudu (Morinda citrifolia
L.). Food Chem. 2006; 94:169–178.