DETERMINATION OF CAROTENOID-PRODUCING BACTERIA ISOLATED IN HON TRE, KIEN HAI DISTRICT, KIEN GIANG PROVINCE

Main Article Content

Lam Hong Bang
Ngoc Nguyen Nhu Huynh
Chon Minh Nguyen

Abstract

Carotenoid is a group of natural pigment which is very diverse in colors, ranging from yellow to red. It is an antioxidant, and is able to increase human immune system. Therefore, the reseach “Determination of carotenoid-producing bacteria isolated in Hon Tre, Kien Hai district, Kien Giang province” aim at isolating hingcarotenoid-producing bacteria to apply in food industry and especially husbandry and aquaculture. The results show that there are 17 carotenoidproducing bacterial strains which were isolated from 6 soil samples and 4 sea water samples collected in Hon Tre, Kien Hai district, Kien Giang province. The colony colors of bacterial strains are ranging from yellow to pink. Average diameters of colonies are approximately from 3 to 5 mm. In terms of morphology, all the bacterial
cells are rod-shaped with different size (2 - 4 µm) x 1 µm. They are Gram-positive, positive - catalase test, and motile. According to results of carotenoid extracted in methanol: chloroform với tỉ lệ 1: 2 v/v from 17 bacterial strains, it is noticed that all the extracts absorb wavelength from 400 – 600 nm. Therefore, these bacterial strains are proved to be likely to produce carotenoid. Three high-producing-carotenoid strains HT 8-1, HT 5-1 and HT 9-3 are 100% similar with Bacillus vietnamensis ANBU6, Bacillus arbutinivorans JPR85 và Bacillus indicus JG-30 .

Downloads

Download data is not yet available.

Article Details

How to Cite
Bang, L., Huynh, N. and Nguyen, C. (2020) “DETERMINATION OF CAROTENOID-PRODUCING BACTERIA ISOLATED IN HON TRE, KIEN HAI DISTRICT, KIEN GIANG PROVINCE”, The Scientific Journal of Tra Vinh University, 1(3), pp. 49-54. Available at: http://journal.tvu.edu.vn/index.php/journal/article/view/225 (Accessed: 16April2021).
Section
Proceeding

References

[1] Britton, G., Liaaen-Jensen, S and Pfander, H. 1995.
Carotenoids Handbook- Section 2: Carotenoids with
a C40 skeleton. Springer Basel AG: 1–50.
[2] Bunariu, M. 2016. Methods of Analysis (Extraction, Separation, Identification and Quantification) of
Carotenoids from Natural Products. J Ecosys Ecograph 6: 193. doi:10.4172/2157-7625.1000193.
[3] Duc, L.H., Fraser, P.D., Tam, N.K and Cutting, S.M.
2006. Carotenoids present in halotolerant Bacillus
spore formers. FEMS Microbiol Lett, 255, 215-224.
[4] El-Agamey, A., Lowe, G.M., McGarvey, D.J.,
Mortensen, A., Phillip, D.M., Truscott, T.G,
et al. 2004. Carotenoids radical chemistry and
antioxidant/pro-oxidant properties. Arch Biochem
Biophys 430: 37–48.
[5] Indra Arulselvi, P., Umamaheswari, S., Ranandkumar
Sharma, G., Karthik, C and Jayakrishna, C. 2014.
Screening of Yellow Pigment Producing Bacterial
Isolates from Various Eco-climatic Areas and Analysis of the Carotenoid Produced by the Isolate. J Food
Process Technol, 5: 1-4.
[6] Khaneja, R., Perez-Fons, L., Fakhry, S., Baccigalupi,
L., Steiger, S., To, E., et al. 2010. Carotenoids found in Bacillus. Journal of Applied Microbiology, 108,
1889-1902.
[7] Khanna, S.K. and Singh, G.B. 1975. Toxicity of
commonly used food colors: A review. J. Sci. Indian
Res, 34: 631-635.
[8] Ramesh, C.H., Mohanraju, R., Murthy, K.N and
Karthick, P. 2017. Molecular characterization of marine pigment bacteria showing antibacterial activity. Indian Journal of Geo Marine Sciences, 46(10):
2081- 2087.
[9] Vílchez, C., Forján, E., Cuaresma, M., Bédmar, F.,
Garbayo, I and Vega, J.M. 2011. Marine carotenoids:
Biological functions and commercial applications.
Mar. Drugs, 9: 319–333.
[10] Vũ Thanh Thảo, Trần Hữu Tâm, Trần Thành Đạo và
Trần Cát Đông. 2011. Khảo sát sự tạo Carotenoid
theo thời gian ở pha sinh dưỡng của một số chủng
Bacillus. Y học Thành phố Hồ Chí Minh, 15(1):211-217.
[11] Yoon, J.H., Kim, I.G., Kang, K.H., Oh, T.K., and
Park, Y.H. 2003. Bacillus marisflavi sp. nov. and
Bacillus aquimaris sp. nov., isolated from sea water
of a tidal flat of the Yellow Sea in Korea. Int J Syst
Evol Microbiol, 53, 1297-1303.